Figure 4.

Nitrogen/carbon/sulfur, thiol, and anion contents in mature seeds of wild-type (Wt) and sultr3 mutant plants. A, Nitrogen/carbon/sulfur seed contents (%) measured by high-performance ionic chromatography. B, Chloride, nitrate, sulfate, phosphate, malate, and citrate seed contents (μmol g⁻¹) measured by high-performance ionic chromatography. C, Cys and glutathione (GSH) contents (nmol g⁻¹) measured by HPLC. Sulfur in the form of sulfate represents 6.3%, 8.9%, 15.7%, 14.2%, 16.3%, and 11.8% of total sulfur in mature seeds of the wild type, sultr3;1, -3;2, -3;3, -3;4, and -3;5, respectively. Bars represent means ± sd of at least four biological replicates. The table in B shows mean ± sd of sulfate percentage in the entire mature seed that is partitioned between the embryo and the surrounding envelopes in the wild type and sultr3;2 after 90 h of seed imbibition (three biological replicates and three technical replicates from each biological replicate). * P < 0.05, ** P < 0.01 (Student’s t test).