Table II. HPLC analysis of chlorophyll and carotenoid contents in wild-type and Golden calli and plantlets.
Values shown are μg g−1 dry weight. Carotenoid composition was measured via diode array HPLC on a minimum of four different calli or stems. Fold change with respect to the wild type is reported for each pigment and for each line. Fold change with respect to the wild type was not calculated in cases in which a metabolite was not detected (indicated by dash).
| Sample | Chlorophyll a | Chlorophyll b | Total Chlorophyll | Violaxanthin + Neoxanthin + Other Xanthophylls | Lutein | β-Carotene | α/β-Xanthophyll | Total Carotene |
| Calli | ||||||||
| Wild type | 8.8 ± 1.7 | 1.7 ± 0.5 | 10.9 ± 2.0 | 10.2 ± 1.9 | 10.5 ± 1.7 | 6.1 ± 1.3 | 1.0 | 48.1 ± 2.5 |
| pK-YBI 41 (E) | 9.1 ± 1.6 | 1.2 ± 0.4 | 10.7 ± 1.7 | 10.6 ± 1.7 | 10.3 ± 3.0 | 8.9 ± 1.1 | 1.0 | 50.8 ± 4.8 |
| Fold change | 1.0 | 0.7 | 0.9 | 1.0 | 1.0 | 1.5 | 1.0 | 1.1 |
| pK-YBI 42 (E) | 8.0 ± 1.4 | 1.1 ± 0.4 | 9.4 ± 1.7 | 10.9 ± 1.9 | 11.0 ± 2.7 | 8.8 ± 1.8 | 1.0 | 49.2 ± 3.3 |
| Fold change | 0.9 | 0.7 | 0.9 | 1.1 | 1.1 | 1.4 | 1.0 | 1.0 |
| pP-YBI 17 (E) | 0.00 | 0.0 | 0.00 | 0.00 | 206.9 ± 38.8 | 605.0 ± 90.22 | – | 811.8 ± 84.8 |
| Fold change | – | – | – | – | 19.8 | 99.0 | – | 16.9 |
| pP-YBI 30 (E) | 0.00 | 0.0 | 0.0 | 0.0 | 302.8 ± 49.8 | 787.3 ± 76.3 | – | 1,090.1 ± 128.4 |
| Fold change | – | – | – | – | 29.0 | 128.9 | – | 22.6 |
| Plantlets | ||||||||
| Wild type | 250.1 ± 29.6 | 102.7 ± 18.1 | 361.3 ± 40.8 | 21.3 ± 4.9 | 42.7 ± 6.5 | 10.3 ± 1.5 | 0.5 | 74.3 ± 8.5 |
| pK-YBI 41 (E) | 173.1 ± 18.2 | 66.7 ± 10.3 | 241.3 ± 37.4 | 24.7 ± 4.0 | 71.2 ± 7.8 | 18.1 ± 1.8 | 0.4 | 113.9 ± 25.8 |
| Fold change | 0.7 | 0.7 | 0.7 | 1.2 | 1.7 | 1.8 | 0.7 | 1.5 |
| pK-YBI 42 (E) | 162.4 ± 28.0 | 53.5 ± 6.4 | 13.7 ± 3.0 | 22.9 ± 5.2 | 74.5 ± 10.3 | 18.4 ± 4.3 | 0.3 | 115.8 ± 30.0 |
| Fold change | 0.7 | 0.5 | 0.7 | 1.1 | 1.7 | 1.8 | 0.6 | 1.6 |
| pP-YBI 17 (E) | 237.3 ± 41.4 | 95.3 ± 16.8 | 334.1 ± 45.5 | 20.0 ± 2.0 | 42.1 ± 6.4 | 11.4 ± 2.1 | 0.5 | 73.5 ± 5.4 |
| Fold change | 1.0 | 0.9 | 0.9 | 0.9 | 1.0 | 1.1 | 1.0 | 1.0 |
| pP-YBI 30 (E) | 255.3 ± 32.8 | 114.1 ± 27.4 | 370.2 ± 52.7 | 22.2 ± 4.0 | 44.7 ± 6.6 | 12.1 ± 2.1 | 0.5 | 79.0 ± 6.8 |
| Fold change | 1.0 | 1.1 | 1.0 | 1.0 | 1.1 | 1.2 | 1.0 | 1.1 |