Figure 4.

Many genes up-regulated in mir159ab are GA regulated in seeds and preferentially expressed in the aleurone. A, Early GA induction in ga1-3 seeds of 16 genes up-regulated in mir159ab. B, Late GA response in ga1-3 seeds of 14 up-regulated genes in mir159ab as determined by qRT-PCR. C, Normalized expression levels for MYB33, MYB65, and the GA-induced genes in the aleurone and embryo. Numbers on top of the bars are absolute expression values in the aleurone. Data for A were obtained from AtGenExpress (Schmid et al., 2005), and data for C were obtained from the Arabidopsis eFP browser (Winter et al., 2007).