Table I. Transcript profiling in mir159ab plants.
Fold change expression levels of select genes found to be misexpressed in mir159ab according to microarray analysis were measured by qRT-PCR in SARs, 28-d-old whole rosettes (WR), 3-d-old imbibed seeds (Seed), and flowers of mir159ab as well as in 28-d-old rosettes of transgenic lines carrying a mMYB33 construct (mMYB; Allen et al., 2007). The rice homologs of these genes that are regulated by GAMYB (Tsuji et al., 2006) in seeds (-S) or anthers (-A) are presented in the table with the percentage of similarity to the Arabidopsis counterparts and the alignment score calculated by the BLAST program in parentheses (National Center for Biotechnology Information; http://blast.ncbi.nlm.nih.gov/Blast.cgi).
| Arabidopsis Genome Initiative Identifier | Gene Title/Putative Function | Fold Change in mir159ab |
mMYBWR | Rice Homolog Up-Regulated by GAMYB |
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| Array | SAR | WR | Seed | Flower | Gene Identifier | Similarity | |||
| Up-regulated genes in mir159ab | |||||||||
| At4g36880 | Cys proteinase (CP1) | 14.40 | 14.93 | 35.30 | −1.31 | 4.98 | 43.16 | AK071495-S | 67% (338) |
| At5g42650 | Allene oxide synthase (AOS) | 9.95 | 4.66 | 1.62 | 2.95 | 1.81 | 2.20 | ||
| At1g75750 | GA-regulated gene 1 (GASA1) | 6.49 | 5.92 | 5.48 | 1.84 | 3.64 | 2.00 | ||
| At4g28040 | Nodulin MtN21 transporter | 5.94 | 12.12 | 10.75 | 15.01 | 2.58 | 6.28 | AK106554-S | 53% (158) |
| AK070604-A | 47% (125) | ||||||||
| At1g44350 | Indole acetic acid-amino acid hydrolase 6 | 5.41 | 2.36 | 1.42 | 5.88 | 2.87 | – | AK110647-A | 64% (145) |
| At3g22880 | Meiotic recombination (DMC1) | 5.32 | 3.66 | 2.68 | 2.66 | 4.50 | 2.22 | ||
| At3g11440 | MYB65 | 4.10 | 3.81 | 4.93 | 2.85 | 5.13 | 2.09 | ||
| At5g49360 | Glycosyl hydrolase (BXL1) | 3.97 | 3.74 | 2.81 | −1.33 | 2.74 | 2.06 | AK072485-A | 43% (112) |
| At1g02640 | Glycosyl hydrolase (BXL2) | 3.96 | 4.07 | 4.50 | −1.12 | 2.93 | 4.34 | AK072485-A | 42% (126) |
| At3g48740 | Nodulin MtN3 transporter | 3.95 | 4.90 | 2.39 | −1.31 | 1.57 | 2.23 | AK103266-A | 75% (263) |
| At5g06100 | MYB33 | 3.69 | 3.75 | 2.85 | 2.96 | 3.45 | 10.72 | ||
| At3g45310 | Cys proteinase | 3.21 | 2.47 | 1.56 | −1.20 | 2.19 | – | AK071495-S | 55% (208) |
| At3g16380 | Poly(A)-binding protein (PAB6) | 3.17 | 5.90 | 1.21 | 1.56 | 1.31 | – | ||
| At3g47010 | Glycosyl hydrolase | 3.12 | 2.67 | 2.14 | 4.50 | 1.70 | 2.10 | AK072485-A | 69% (654) |
| At3g14067 | Subtilase protease | 2.58 | 1.87 | 1.43 | −1.23 | −1.41 | – | AK105112-A | 52% (437) |
| AK106823-A | 49% (280) | ||||||||
| At3g28220 | Metalloproteinase | 2.45 | 2.93 | 1.37 | 20.93 | 1.28 | – | ||
| At2g03710 | MADS box protein (AGL3) | 2.35 | 2.94 | 1.82 | 3.58 | 1.06 | 1.90 | ||
| At1g58270 | Metalloproteinase | 2.11 | 1.93 | 1.46 | −1.22 | 1.57 | – | ||
| At5g27360 | Sugar porter (SFP2) | 2.05 | 4.14 | 1.18 | 3.01 | −1.10 | – | AK069132-A | 42% (144) |
| At5g09220 | Amino acid permease (AAP2) | 2.05 | 2.35 | 1.38 | 1.43 | −1.30 | – | AK106814-A | 61% (346) |
| AK067118-S | 44% (117) | ||||||||
| Down-regulated genes in mir159ab | |||||||||
| At5g55450 | Protease inhibitor | −7.15 | −1.48 | −2.34 | −1.08 | −1.33 | −3.38 | ||
| At5g28640 | SSXT-related protein (AN3) | −2.36 | −1.45 | 1.02 | −1.51 | −1.10 | −1.35 | ||
| At2g19590 | 1-Aminocyclopropane-1-carboxylate oxidase 1 (ACO1) | −2.35 | −8.14 | −4.30 | −1.29 | −1.41 | −6.60 | AK058296-A | 61% (218) |