Figure 4.

Cuticle and cell wall permeability of sitiens and the wild type. A, Extraction of chlorophyll from equal amounts of wild-type and sitiens intact leaves for 0.5 to 4 h in 80% ethanol at room temperature. B, Total chlorophyll extracted from leaves of the wild type, sitiens, and sitiens complemented with ABA by spraying 100 μm ABA in 0.05% ethanol until runoff twice per week during their development at different time intervals with sd. C, Leaf discs excised from the fourth leaf of 5-week-old wild-type and sitiens plants floating on their adaxial side on a 0.05% toluidine blue solution in water for 1 h. sitiens plants were complemented with ABA by spraying 100 μm ABA in 0.05% ethanol until runoff twice per week during development. As a control, a mock solution containing 0.05% ethanol was used. Biological replicates of the experiments produced similar results.