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Pectinase treatments of wild-type and sitiens leaf discs. A, Leaf discs excised from fifth leaves of wild-type and sitiens plants floating at room temperature on 1 mL of pectinase (Pectinex Ultra SPL; Sigma-Aldrich) solutions at different enzyme concentrations (units mL−1). Photographs were taken after 3 d. B, Quantification of the leaf disc surface area with the image-analysis software APS Assess 2.0. Two biological replicates gave similar results. WT, Wild type.
