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. 2010 Jul 29;154(2):820–832. doi: 10.1104/pp.110.157560

Figure 2.

Figure 2.

CTF7 is the Arabidopsis cohesion establishment factor. A, Arabidopsis CTF7 exhibits acetyltransferase activity. Lanes 1 and 3, Molecular mass markers; lane 2, Coomassie Brilliant Blue-stained SDS-PAGE gel of affinity-purified CTF7-MBP; lane 4, Western blot of affinity-purified CTF7-MBP treated with anti-acetyl-Lys antibodies. Full-length CTF7-MBP is marked with arrowheads. A cleaved version of CTF7 is marked with arrows, and MBP is labeled with a star. B, Arabidopsis CTF7 can substitute for S. cerevisiae CTF7. Cultures of wild-type (WT) and temperature-sensitive ctf7-203 cells containing the pAS2 control plasmid or an Arabidopsis CTF7 complementation plasmid were grown on the appropriate selection plates at either 28°C or 33°C.