Figure 2.

Transient expression of amiRNAs targeting specific circadian clock components recapitulates loss-of-function mutant phenotypes. A, Bioluminescence traces from protoplast cells transfected as described in Figure 1 using the CCA1-LUC reporter and the indicated gene-specific amiRNA in constant red light (R). B, As in A but under constant blue light (B; 5 μmol m⁻² s⁻¹). Data represent mean ± SEM (n = 6). Similar results were obtained in two independent trials.