Figure 3.

Transient expression of amiRNA reduces expression of the endogenous target genes. A, Schematic of the GI, ELF3, and ZTL transcripts with the amiRNA targeted site (vertical bars) and the PCR-amplified region (horizontal black bars) indicated for each. B, Relative expression of GI, ELF3, and ZTL from protoplasts transfected with control amiRNA or the gene-specific amiRNA indicated and harvested at ZT12. Expression levels were determined by real-time qPCR and normalized to ACTIN2 (ACT2). Data represent mean ± SEM (n = 3).