Table I. Estimates of free-running period in transiently transfected Arabidopsis protoplasts under constant light.
R, Red light; B, blue light.
| Genotype | Effector (AmiRNA) | Reporter (Luciferase) | Light | Period | Figure |
| h | |||||
| Col | – | CCA1-LUC | R | 25.8 ± 0.1 (6/6) | Figure 1C |
| elf3-1 | – | CCA1-LUC | R | N.D. (0/6) | Figure 1C |
| toc1-101 | – | CCA1-LUC | R | 24.4 ± 0.4 (6/6) | Figure 1C |
| ztl-3 | – | CCA1-LUC | R | 27.9 ± 0.2 (6/6) | Figure 1C |
| Col | – | GI-LUC | R | 25.8 ± 0.1 (6/6) | Figure 1D |
| elf3-1 | – | GI-LUC | R | 29.7 ± 0.0 (2/6)a | Figure 1D |
| toc1-101 | – | GI-LUC | R | 18.4 ± 2.2 (6/6)b | Figure 1D |
| ztl-3 | – | GI-LUC | R | 29.2 ± 0.3 (3/5) | Figure 1D |
| Col | Control | CCA1-LUC | R | 26.3 ± 0.1 (6/6) | Figure 2A |
| Col | ELF3 | CCA1-LUC | R | 31.1 ± 1.6 (6/6) | Figure 2A |
| Col | GI | CCA1-LUC | R | 24.0 ± 0.4 (6/6) | Figure 2A |
| Col | ZTL | CCA1-LUC | R | 28.8 ± 0.1 (6/6) | Figure 2A |
| Col | Control | CCA1-LUC | B | 26.5 ± 0.1 (6/6) | Figure 2B |
| Col | ELF3 | CCA1-LUC | B | N.D. (0/6) | Figure 2B |
| Col | GI | CCA1-LUC | B | 23.4 ± 0.2 (6/6)b | Figure 2B |
| Col | ZTL | CCA1-LUC | B | 30.8 ± 0.2 (5/5) | Figure 2B |
| Col | Control | CCA1-LUC | R | 25.8 ± 0.3 (6/6) | Figure 4A |
| Col | CKBfam | CCA1-LUC | R | 29.6 ± 0.1 (6/6) | Figure 4A |
a
Period is not representative of the multiple trials but an artifact of the period estimation program that returned circadian range period estimates in two instances. Period estimates were generated using BRASS (http://www.amillar.org) from bioluminescence readouts shown in figures indicated. Data are shown as variance weighted mean period ± SEM (rhythmic samples/total samples). –, Without amiRNA plasmid; N.D., not detectable.
b
Period estimates were determined using a 60-h window (from 24–84 h).