Figure 4.

Sugar levels and sugar compartmentation in Arabidopsis seedlings grown in liquid culture. A, Sugar content in seedlings from 7-d-old wild-type (Wt) and 35S:tmt1;1-2 plants grown in liquid medium supplemented with 5% Glc. Dark gray bars represent wild-type samples, white bars represent tmt1-2::tDNA, striped bars represent 35S:tmt1;1-2 line 1, diagonal striped bars represent line 4, and light gray bars represent line 10. Data represent means of three independent experiments ± se. FW, Fresh weight. B, Seedlings were grown in liquid medium supplemented with 5% Glc. Sugar compartmentation was analyzed via nonaqueous fractionation. Dark gray bars represent the vacuole, white bars represent the plastid, and light gray bars represent the cytosol. Data represent means of two independent experiments ± se, each consisting of three individual plants. Note that in some samples, we calculated more than 100% of total sugar due to se. Asterisks above the bars report the results of a significance test (Student’s t test) for differences between the TMT mutants and the wild type: *** P < 0.005, ** P < 0.01.