Figure 6.

Respiratory activity, intracellular sugar compartmentation, and gene expression in Arabidopsis wild-type (Wt) and TMT mutant plants. A, Respiration of [¹⁴C]Glc in 3-week-old Arabidopsis seedlings. Plants were grown on soil under standard conditions. Dark gray bars represent wild-type samples, white bars represent tmt1-2::tDNA, striped bars represent 35S:tmt1;1-2 line 1, diagonal striped bars represent line 4, and light gray bars represent line 10. Data given represent means of four individual experiments, each with five replicate samples ± se. B, qRT-PCR was used to validate the expression level of CAB1 and NR1 in soil-grown Arabidopsis leaves. Data represent means of three independent experiments ± se. Asterisks above and below the bars report the results of a significance test (Student’s t test) for differences between the TMT mutants and the wild type: *** P < 0.005, ** P < 0.01, * P < 0.5.