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. Author manuscript; available in PMC: 2011 Sep 3.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Aug 1;399(4):555–559. doi: 10.1016/j.bbrc.2010.07.109

Figure 1.

Figure 1

Characterization of the recombinant EphA4 LBD. (a) The SEC elution profile of the EphA4 LBD after final purification. The fractions under the major peak were resolved on SDS-PAGE and correspond to the pure protein. (b) The purified EphA4 LBD retains its biological activity. The SDS-PAGE shows that the EphA4 LBD forms a specific complex with the Fc-tagged ephrin-A5 ligand (ephrin-A5-Fc) that can be pulled down by Protein-A Sepharose beads (lane 1). The EphA4 LBD by itself does not bind to the beads (lane 2).