Table II.
Primers and probes for quantitation of mRNA by Q-PCR for rabbit PPIA control, β2-microglobulin (B2M) and PAK1a
| PPIA control | |
| PPIA forward | 5′ CAACACAAATGGCTCCCAGTT 3′ |
| PPIA reverse | 5′ CATGGCTTCCACAATGCTCAT 3′ |
| PPIA probe | 5′ ATCTGCACTGCCAAGAC 3′ |
| Beta-2-microglobulin (B2M) | |
| B2M forward | 5′ TTGTTCCCCTGCCTGGAGT 3′ |
| B2M reverse | 5′TGGATGACGAGAGTACACTTGAACAT 3′ |
| B2M probe | 5′ CCAGCGTGCTCCG 3′ |
| PAK1 | |
| PAK1 forward | 5′ GAAGGCCAGATTGCAGCTGT 3′ |
| PAK1 reverse | 5′ CGAATGCAGAAACTCCAGAGC 3′ |
| PAK1 probe | 5′ AGGTCTGGGCGGATG 3′ |
a
The total volume of PCR reaction was 25 μl and the PCR conditions were as follows: 50°C for 2 min, 95°C for 10 min, followed by 40 cycles of 95°C, 15 s for denaturation and 60°C, 1 min for annealing and extension.