Figure 6.

Time course of glial fibrillary acidic protein (GFAP) mRNA expression in control and darglitazone-treated ob/+ and ob/ob mice: In situ hybridization. Astrocytes response was measured by in situ hybridization using GFAP ³⁵S-labeled riboprobe. Darglitazone did not significantly change the GFAP expression in ob/+ mice compared with untreated group. The pattern of GFAP expression in the darglitazone-treated ob/ob mice was comparable with both untreated and darglitazone-treated ob/+ mice and was consistent with minimal damage. The expression pattern seen in the control ob/ob mice was predominantly penumbral and reflected pronounced ischemic damage.