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letter
. 2009 Oct 14;30(2):267–272. doi: 10.1038/jcbfm.2009.217

Figure 2.

Figure 2

BBB permeability and MMP-9 expression are increased in obese mice after MCAo. BBB permeability was assessed by immunostaining for IgG. (A) Representative sections from lean and obese ob/ob mice 24 h after MCAo illustrate marked increased in the intensity and distribution of IgG immunoreactivity in ob/ob mice in the cortex and striatum indicating greater BBB disruption in obese mice. (B) Representative photomicrographs of MMP-9 expression in the brain of lean and obese ob/ob mice 24 h after MCAo. Increase in MMP-9 immunoreactivity was observed in the striatum and cortex of ob/ob mice. (C) Multi-labelling immunofluorescence shows colocalisation of laminin-positive blood vessels (red) and MMP-9 expression (green) in ob/ob mice indicating that the majority of MMP-9 is localised to cerebral blood vessels. Scale bars, 100 μm (B) and 25 μm (C).