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. 2009 Oct 28;30(2):323–335. doi: 10.1038/jcbfm.2009.216

Figure 2.

Figure 2

Effects of postnatal thyroid hormone deficiency on microvessels in the dentate gyrus. Postnatal hypothyroidism was induced with PTU in the drinking water from birth to P21. Immunohistochemistry staining of mouse anti-RECA (microvessel marker) showed visible changes of microvessels in the dentate gyrus at P21 in the PTU-treated rats (A and B), with full recovery at P90 (C and D). Quantitative analysis of dentate gyrus microvessels was based on several parameters: branching points (E), microvessel density (F), and microvessel diameter (G) in 40-μm-thick, sagittal sections. At P21, PTU treatment resulted in decreased branching points, decreased microvessel density, and enlarged diameter of microvessel. However, by P90, these changes were no longer apparent. Data are presented as mean±s.e.m. from P21 (n=8) and P90 rats (n=16, 8 male and 8 female). Two-sample equal variance t-test was applied, ***P<0.001, **P<0.01. Scale bar in panel A=50 μm.