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. 2009 Oct 28;30(2):323–335. doi: 10.1038/jcbfm.2009.216

Figure 5.

Figure 5

Thyroid hormone receptor (TR) expression in microvessels of the cortex and REB4 cells. (A) Purely isolated microvessels from the rat cortex. (B) TR expression in isolated microvessels determined by RT-PCR. (C) Immunohistochemistry staining of RBE4 cells against TR-β1 (orange signal) and RECA (green signal) revealed intense expression by endothelial cells (DAPI nuclear staining in blue). Immunostaining against TR-α1 and TR-β2 obtained similar results (data not shown). (D) TR expression in RBE4 cells determined by RT-PCR. Scale bar in panel A=250 μm and in panel C=25 μm.