Figure 4.

Isolated BINCs expressed mRNAs encoding a variety of neuroprotective factors at significant levels as shown by quantitative real-time RT-PCR. The vertical axes of the graphs denote the expression level of the mRNA encoding each factor as the percentage of β-actin mRNA expression level. Total RNA was extracted from BINCs shortly after their isolation from ischemic brains at 7 dpr. The levels of the expression by BINCs were compared with primary cultured astrocytes (AC), microglia (MG), and peritoneal macrophages (PM). Note the high expression level of IGF-1 in MG and BINCs, which reached around 30% of the β-actin mRNA level. Among the other factors, HGF mRNA expression was higher in BINCs than in other cells. Data of triplicate experiments from four separate cultures of each cell type are expressed as mean±s.e.m.