Figure 5.

Analysis of the adhesion defects in β3 integrin-null brain endothelial cells (BEC). Wild-type or β3 integrin-null BEC were plated onto the extracellular matrix (ECM) proteins, collagen I, collagen IV, fibronectin, laminin, or vitronectin. After 3 h, photographs were taken. Scale bar=50 μm. Note that wild-type BEC attached and spread on all ECM proteins tested. In contrast, β3 integrin-null BEC showed marked adhesive defects on vitronectin and collagen I, and a mild defect on collagen IV, though adhesion to fibronectin and laminin was no different from wild-type cells.