Figure 6.

Quantification of immunofluorescence signals for Connexin (Cx) 43. As described in Figure 3, confocal pictures from double immunofluorescence were taken and connexin immunosignals were quantified from segmented images. Quantification of vesicle sizes of Cx 43 immunoreactivity signals revealed a significant shift toward larger size plaques 6 hours after ultrasound treatment in insonicated areas compared with control hemispheres. Small-sized plaques were found to be significantly reduced in insonicated areas after 6 hours. As for Cx 36, this effect disappeared after 24 hours. Black bars, albumin-positive; gray bars, albumin-negative.