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Matrix synthesis rates during short-term loading. Constructs were cultured in basal or chondrogenic medium for 4 or 12 days and loaded intermittently (3 h on–3 h off) for 24 h. (A) Protein and (B) proteoglycan (PG) synthesis rates were measured during the 24 h loading period by incorporation of 3H-proline and 35S-sulfate, respectively. The total (C) sGAG and (D) DNA contents were also measured in the constructs. n = 6/condition, *p < 0.05 with unloaded.
