FIG. 6.

Extracellular matrix composition and organization. Portions of the fibrin gel constructs were formalin fixed, paraffin embedded, and sectioned to 5 μm. (A) Cross sections from day 21 constructs from FS, unloaded (0%), or cyclically loaded (+10%) groups were stained for sGAG with Safranin-O and counterstained with Fast Green. (B) Transverse sections from day 14 samples were stained with Picrosirius Red. Arrows indicate the long axis and loading direction of the constructs. (C) Type I collagen (green) and type II collagen (red) were detected by immunofluorescence staining of day 21 cross sections, and inset shows the absence of staining without the primary antibody. All scale bars are 50 μm.