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Toroid diameter and lumen diameter can be controlled by micromold design. Monodispersed cells (2 × 106) were seeded onto agarose micromolds containing toroid-shaped recesses of varying dimensions. The width of the circular tracks were the same (400 μm), but the diameter of the peg varied, 400, 600, 800, or 1000 μm (A–D). Forty-eight hours after seeding, monodispersed cells self-assembled multicellular toroids that contracted around the peg. Scale bar is 200 μm.
