Table 2. Homogenate binding studies: B_max and K_d for HABs and LABs with [³H]PBR28 and [³H]PK11195.

Donor	[3H]PK11195: Kd (nmol/L) and Bmax (fmol per mg protein)		[3H]PBR28: Kd (nmol/L) and Bmax (fmol per mg protein) using a single-site model		[3H]PBR28: Kd (nmol/L) and Bmax (fmol per mg protein) for two-site model, where appropriate
	Bmax	Kd	Bmax	Kd	Total Bmax (for both sites)	kd (High-affinity site)	Kd (Low-affinity site)
HABs
1	4998	16.17	1432	2.49
2	6223	32.39	2410	2.23
3	3003	29.36	1550	2.75
4	3908	51.72	1305	1.34
7	6263	42.79	1879	2.30
8	5217	32.03	1296a	2.22a	1479	1.1	21
9	4693	32.51	977a	2.35a	2337	1.48	374
10	b	b	3000	2.36
11	2599	10.18	1199a	3.53a	1709	1.48	69
12	2018	9.23	1305a	2.86a	1917	1.15	76
Mean±s.d.	4325±1540 (n=9)	28.5±14.35 (n=9)	1929±657 (n=6)	2.2±0.48 (n=6)	1861±365 (n=4)	1.3±0.2 (n=4)	135±161 (n=4)
LABs
5	5843	27.44	1440	35.36
6	2765	18.53	611	32.42
20	5725	50.59	691	46.85
21	5174	18.83	985	89.64
22	5808	37.79	1243	57.58
Mean±s.d.	5063±1313 (n=5)	30.6±13.65 (n=5)	994±353 (n=5)	52.4±23.13 (n=5)
T-test	P=0.38	P=0.79	P=0.02	P<0.001

HABs, high-affinity binders; LABs, low-affinity binders.

^aData did not fit a single-site model and was therefore excluded from calculation of mean B_max and K_d.

^bCurve did not reach full plateau, and therefore accurate values for K_d and B_max could not be calculated.