Table 2.

Differentially Pretreated Smooth Muscle Cell Cultures That Were Used as Controls to Compare with the Endothelial Cell–Smooth Muscle Cell Cocultures

Desired cell phenotype	Medium	Experimental setup
Hyperplastic SMCs	SMGS-supplemented M231 (SMC growth medium)	Negative control
Contractile SMCs	SMDS-supplemented M231 (SMC differentiation medium)	Test for differentiation indices (e.g., SMA, calponin)
More contractile SMCs	2.5TGF-β1-treated SMCs or 5TGF-β1-treated SMCs (enhanced SMC differentiation medium)	Test for differentiation indices (e.g., SMA, calponin)

SMA, smooth muscle actin.