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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Mol Cancer Ther. 2010 Aug 24;9(9):2545–2557. doi: 10.1158/1535-7163.MCT-10-0337

Figure 1. Functional p53 expression is required for maximal induction of apoptosis by JNJ-26854165.

Figure 1

(A) Cells were treated with 5 μM JNJ-26854165 for the indicated times, and the Annexin V-positive fractions were measured by flow cytometry. (B–C) Parental and lentivirally transduced OCI-AML3 cells [virus encoding either scrambled shRNA (shC) or p53-specific shRNA (shp53)] were incubated with the indicated concentrations of JNJ-26854165 for 48 hours, and CMXRos retention (B) or the number of viable cells (C) were assessed. Results are expressed as the percentage of the numbers of viable cells in an untreated group. Asterisk (*) indicates significance at P < .05. p53 expression was determined by western blot analysis in untreated or γ-irradiated cells 5 hours after 6 Gy irradiation. (D) Cells were incubated with 5 μM JNJ-26854165 for 48 hours, and stained for DNA content. Cell cycle distribution was analyzed using ModFit LT software. Results are representative of three independent experiments.