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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Metab Eng. 2010 Apr 20;12(4):332–340. doi: 10.1016/j.ymben.2010.04.001

Figure 6. GK-overexpressing cell line GK2 has higher GR transcription factor activity than the wild type (WT).

Figure 6

WT (open circles) and GK2 (filled circles) cell lines were assayed for glucocorticoid receptor transcription factor activity by measuring the expression level of known GR-responsive gene PEPCK to glucocorticoid agonist dexamethasone for (a) 1 h and (b) 2 h. Gene expression levels are relative to dexamethasone-untreated controls (0 nM) for the same line. ‘*’ indicates p < 0.05 between different dexamethasone dosages on the same cell line. ‘**’ indicates p < 0.05 between lines GK2 and WT for the same dexamethasone dosage. Gene expression was assayed by quantitative RT-PCR, as described in Methods.