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. Author manuscript; available in PMC: 2010 Oct 4.
Published in final edited form as: Nat Rev Genet. 2009 Jan;10(1):57–63. doi: 10.1038/nrg2484

Figure 4. Poly(A) tags from RNA-Seq.

Figure 4

A region containing two overlapping transcripts (ACT1, from the actin gene, and YFL040W, an uncharacterized ORF) from the Saccharomyces cerevisiae genome is shown. Arrows point to transcription direction. The poly(A) tags from RNA-Seq experiments are shown below these transcripts, with arrows indicating transcription direction. The precise location of each locus identified by poly(A) tags reveals the heterogeneity in poly(A) sites, for example, ACT1 has two big clusters, both with a few bases of local heterogeneity. The transcription direction revealed by poly(A) tags also helps to resolve 3′-end overlapping transcribed regions18.