Figure 2.

Evoked release is reduced in phospholipid-binding mutants of both C₂ domains. A, Representative traces recorded from larval muscle fiber 6. Each trace represents the mean of 30 consecutive sweeps from the same muscle fiber. B, Compared with P[syt^WT], the mean EJP amplitude of all P[syt^RQ] lines was significantly decreased (*, **p < 0.0001, 1-way ANOVA; P[syt^WT], n = 16; P[syt^A-RQ] line 7, n = 12; P[syt^A-RQ] line 8, n = 14; P[syt^B-RQ] line 3, n = 16; P[syt^B-RQ] line 4, n = 13). Additionally, the evoked responses of the P[syt^B-RQ] lines were significantly lower than those of the P[syt^A-RQ] lines (*, **p < 0.01), but no differences were found between P[syt^A-RQ] lines 7 and 8, or between P[syt^B-RQ] lines 3 and 4 (p > 0.2).