Figure 3.

mEJP frequency is increased in phospholipid-binding mutants of both C₂ domains. A, Mean mEJP frequencies of each mutant and the transgenic control. P[syt^WT], n = 55 fibers; P[syt^A-RQ] line 7, n = 7 fibers; P[syt^A-RQ] line 8, n = 17 fibers; P[syt^B-RQ] line 3, n = 27 fibers; P[syt^B-RQ] line 4, n = 12 fibers. Compared with P[syt^WT], all genotypes had an increased mEJP frequency (p < 0.05). No significant difference was detected between P[syt^A-RQ] and P[syt^B-RQ] (p > 0.05), between P[syt^A-RQ] lines 7 and 8 (p > 0.1), or between P[syt^B-RQ] lines 3 and 4 (p > 0.1). *p < 0.0001, **p < 0.05, compared with P[syt^WT]. B, Frequency distribution curves of mEJP amplitudes calculated from 1000 individual events per transgenic line in 0.2 mV bins. P[syt^WT], P[syt^A-RQ] line 8, and P[syt^B-RQ] line 3 are shown.