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Quantitative PCR amplification of genomic DNA from unrelated male patients, using Beggs set of primers ( Bc) amplifying exons 47–60–52 as described in patients and methods and PCR products were electrophoresed on 1.5%nusceive gels containing ethidium bromide DNA samples amplified using Beggs (Bc) set of primers amplifying exons 47–60–52: Lane 1–2:case 28, male BMD patient showing duplication in band 52, Lane 3–4: control male, Lane 5–6: control female.
