Figure 1.

Representative flow cytometry gating strategy. Mononuclear cells were selected based on forward and side scatter characteristics (left panel). Panel (A): Cells were incubated with BODIPY-aminoacetaldehyde in the presence (center panel) or absence (right panel) of diethylaminobenzaldehyde, a specific aldehyde dehydrogenase (ALDH) inhibitor (y axis = side scatter in all panels). Panel (B): Cells were incubated with antibodies to CD34, CD133, and VEGFR-2 or their IgG controls, and the expression of individual markers was determined. For cells expressing combinations of markers (eg, CD133 and CD34), the CD133 gate was dragged into the CD34 gate to obtain dual expressing cells. DEAB = diethylaminobenzaldehyde; FITC = fluorescein isothiocyanate.