Fig. 3.

Treatment of malignant cells with reverting agents results in organized structures with proper polarity. Confocal microscopy of labeled nuclei, F-actin, β-catenin, β4 integrin, and laminin-5 reveals that while malignant cells form dense and disorganized clusters (middle column) marked by diffuse staining of F-actin, β-catenin, and integrin β4, and fail to deposit a basement membrane (BM), treating malignant cells with any of several reverting agents (β1 integrin targeting monoclonal antibody AIIB2 shown, right column) results in normalized clusters (compare to non-malignant cells, left column). These polarized clusters exhibit cortically organized F-actin, β-catenin concentrated at cell–cell junctions, basally localized β4 integrin, and basally secreted BM. This figure was reproduced, with permission from Elsevier, from Weaver et al. (2002)