Figure 3. Memory B cell sorting strategies.

Subsets of human B cells (CD45⁺CD19⁺) were sorted from BM and spleens of vaccinated HIS mice. The strategy 1 (upper part) was used on 3 responder HBsAg-vaccinated animals and gave rise to the following B cell subsets: (i) CD27^hiCD38^hi, (ii) CD27⁺CD38^lo/intIgD⁺, and (iii) CD27⁺CD38^lo/intIgD⁻. The strategy 2 (lower part) was used on 3 responder TT-vaccinated animals and gave rise to the following B cell subsets: (iv) CD27⁺IgM⁺IgG⁻, and (v) CD27⁺IgM⁻IgG⁺. The respective frequency and name (from i to v) of each sorted B cell subsets is indicated in the corresponding flow cytometry dot plot.