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. 2010 Oct 4;5(10):e13137. doi: 10.1371/journal.pone.0013137

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Becker et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) After obtaining monoclonal TT-specific B cell lines, B cell clones were tested for cross-reactivity against unrelated antigens from HBV or RSV in an ELISA test. (B) The direct binding of phycoerythrin-labeled TT on TT ELISA positive B cell clones was determined by flow cytometry, and 3 sub-types of clones are observed: high, intermediate and low binding clones. Values in the histograms show the percentage of cells within the indicated gate.