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. Author manuscript; available in PMC: 2011 Nov 10.

Published in final edited form as: Neuroscience. 2010 Aug 21;170(4):1261–1269. doi: 10.1016/j.neuroscience.2010.08.019

50nM estradiol protects female but not male neurons from glutamate toxicity. At 10DIV, male and female primary cortical neurons were exposed to 0.005% ethanol or 50nM estradiol for 5 minutes prior to a five minute exposure to 5mM glutamate. After 1 wash in complete Neurobasal media, cells received fresh media and were returned to the incubator for 24 hours. A. Representative images from the Live/Dead assay (Invitrogen). Living neurons are stained green by Calcein AM. Dead cells are stained red by the ethidium bromide homodimer. B. 5 minute pre-exposure to 50nM estradiol protects female neurons (***Bonferroni t _{(glutamate vs. estradiol+ glutamate)} =3.911, p<0.001) but not male neurons from glutamate toxicity (n=5).

50nM estradiol protects female but not male neurons from glutamate toxicity. At 10DIV, male and female primary cortical neurons were exposed to 0.005% ethanol or 50nM estradiol for 5 minutes prior to a five minute exposure to 5mM glutamate. After 1 wash in complete Neurobasal media, cells received fresh media and were returned to the incubator for 24 hours. A. Representative images from the Live/Dead assay (Invitrogen). Living neurons are stained green by Calcein AM. Dead cells are stained red by the ethidium bromide homodimer. B. 5 minute pre-exposure to 50nM estradiol protects female neurons (***Bonferroni t _{(glutamate vs. estradiol+ glutamate)} =3.911, p<0.001) but not male neurons from glutamate toxicity (n=5).