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. Author manuscript; available in PMC: 2011 Nov 10.

Published in final edited form as: Neuroscience. 2010 Aug 21;170(4):1261–1269. doi: 10.1016/j.neuroscience.2010.08.019

ICI 182, 780 blocks estrogen neuroprotection in female neurons. Male and female neurons were exposed to (A) 0.01% ethanol or (B) 1uM ICI for 15 minutes prior to a 5 minute exposure to 0.001% ethanol or 10nM estradiol. Neurons we subsequently exposed to 5mM glutamate for 5 minutes. (A) Estradiol (10 nM) protected female neurons but not male neurons from glutamate toxicity in ethanol pretreated neurons (***Bonferroni post hoc t _{(glutamate vs. estradiol+ glutamate)} =4.977, p<0.001, n=4). (B) ICI pretreatment blocked estradiol protection in female neurons (n=4).

ICI 182, 780 blocks estrogen neuroprotection in female neurons. Male and female neurons were exposed to (A) 0.01% ethanol or (B) 1uM ICI for 15 minutes prior to a 5 minute exposure to 0.001% ethanol or 10nM estradiol. Neurons we subsequently exposed to 5mM glutamate for 5 minutes. (A) Estradiol (10 nM) protected female neurons but not male neurons from glutamate toxicity in ethanol pretreated neurons (***Bonferroni post hoc t _{(glutamate vs. estradiol+ glutamate)} =4.977, p<0.001, n=4). (B) ICI pretreatment blocked estradiol protection in female neurons (n=4).