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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2010 Jun 15;122(4):219–231. doi: 10.1016/j.jsbmb.2010.06.006

Figure 3. Temporal Effects of Luteolin on EGFSP and CCP Gene Expression in PC-3 Cells.

Figure 3

PC-3 cells were plated and grown and described in methods. Twenty four hours following plating, the cells were treated with 17.5 µM luteolin in 2 µL of EtOH. At various times following treatment (1, 8, 16, 24, 48, 72 hours) the cells from time matched controls or luteolin treated cells were harvested and RNA was prepared and analyzed by qPCR. Relative Expression values represent the mean ± SEM for a minimum of three independent RNA sets from time matched EtOH controls and luteolin treated PC-3 cells, each normalized to 18S RNA as described in detail [30]. To simplify the figure, the control for all time points was represented at 100%. Statistical analyses were performed on quadruplicate aliquots from each of three replicated experiments for each luteolin sample time point and its corresponding control.