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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2010 Jun 15;122(4):219–231. doi: 10.1016/j.jsbmb.2010.06.006

Figure 5. Assessment of Luteolin Effects on PC-3 Cell Proliferation by Flow Cytometry.

Figure 5

PC-3 cells were grown and plated as described in Methods. Twenty-four hours following plating, the cells were treated with two doses of luteolin (17.5 µM; 35 µM) or Gefitinib (22 µM; 44 µM) alone or in combination (as indicated in each panel) and 24 hours following treatment, the cells were collected, stained with propidium iodide and analyzed by flow cytometry (see Methods for details) as previously described by our laboratory [26]