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Phase contrast and immunofluorescence images of NSCs after incubation in FGF2-containing media for 5 days. NSCs grown on all surfaces proliferated and expressed Nestin (shown in green). Cells on EggPC and 40% GRGDSP surfaces formed loosely attached aggregates, whereas cells on laminin, and to a lesser extent, 20% bsp-RGD(15), grew as a monolayer of single cells attached to the surface. Scale bar: 100 µm.
