Fig 2.
Growth and cellular zinc content of adcR mutants. (a) Growth curves of adcR parent, ΔadcR, adcR C30A, adcR H108Q, adcR H111Q, and adcR H112Q strains of S. pneumoniae in the presence of 200 μM ZnSO4. (b) Cellular zinc concentration in bacteria growing exponentially (OD620 = 0.1 to 0.3). The average of three biological replicates are shown with SEMs (P < 0.05). See Materials and Methods for ICP-MS and calculation of cellular concentration.