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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Chem Biol Drug Des. 2010 Aug 30;76(4):293–304. doi: 10.1111/j.1747-0285.2010.01021.x

Figure 3.

Figure 3

Peptide immunogens synthesized, using the PAO receptor binding domain (RBD) sequence as a template. Boxed residues indicate substitutions from the native strain PAK RBD sequence, into the native strain PAO RBD sequence of the peptides. Boxed residues also indicate native strain PAK specific residues while the circled residues indicate native strain PAO specific residues; all other residues are identical in PAK and PAO RBDs. Immunogen A (PAO(128–144) native sequence), Immunogen B (PAO(128–144) P135E/M136Q), Immunogen C (PAO(128–144) P135E/M136Q/T138I), Immunogen D (PAO(128–144) T132D/P135E/M136Q/T138I), and Immunogen E (PAO(128–144) K130T/T132D/P135E/M136Q/T138I). −OH denotes a C-terminal carboxyl group. The peptides were covalently attached to keyhole limpet hemocyanin (KLH), via the α-amino group, for immunization as described in the methods.