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Competitive ELISAs using antibody A, which is specific for PAO pilin. In Panels A, B, and C, PAO pilin is the competitor. In Panel A, PAO pilin is plated; in Panel B, PAO (KSK) mutant pilin is plated and in Panel C PAK (ADN) mutant pilin is plated. IC50, the concentration of pilin protein resulting in 50% inhibition of antibody binding in the competitive ELISA.
