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Competitive ELISAs using antibody B1, which is cross-reactive to PAO pilin (panel A) and PAK pilin (Panel B). Antibodies which bind to PAO pilin (Panel C) or PAK pilin (Panel D) can be readily competed by PAK mutated pilin, PAK (ADN). IC50, the concentration of pilin protein resulting in 50% inhibition of antibody binding in the competitive ELISA.
