Figure 1.
Enzymatic activity of bee-venom PLA2 (±5%) on L-DPPC in mixed aggregates of sodium taurocholate + L-DPPC +D-DPPC as a function of the interface substrate concentration, [L]s for five surface dilution series’. The concentration ratio of bile salt to total DPPC is constant at six. The total DPPC concentration varies from 0.002 to 0.020 M. Within each of the series, variation in [L]s is achieved by varying the L-DPPC to D-DPPC ratio. [L]s was calculated from measured values of aggregation number and interface hydration according to the methods in the Appendix. The lines are fits to eq. 3.