Figure 1. Inhibited CHS responses in mice treated with placental extract implantation.
BALB/c mice underwent implantation with 3 mg placental extract (∉) or sham operation (⊄ and Υ) as described in the materials and methods. After 3 days, the mice were sensitized with 0.5% DNFB (∉ and Υ) or vehicle alone (⊄; non-sensitized control), and this procedure was repeated 24 hrs later. Five days after the first sensitization, the mice were challenged with 0.2% DNFB on earlobe, and its thickness was measured everyday. (A) Net increase of earlobe thickness at the indicated time points are shown as the mean +/− SEM (n=5). **P< 0.01, *P< 0.05 between CHS mice treated with placental extract implantation and those with sham operation. (B) Two days after DNFB challenge, earlobe tissues were harvested, processed, and stained with H&E. Representative fields of the tissues from 3 repeated experiments are shown.