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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Int Immunopharmacol. 2010 Jul 7;10(10):1177–1184. doi: 10.1016/j.intimp.2010.06.024

Figure 3. Preventive effects of placental extract on memory CHS responses.

Figure 3

BALB/c mice underwent implantation with 3 mg placental extract (∉) or sham operation (⊄ and Υ). After 3 days, the mice were sensitized with 0.5% DNFB (∉ and Υ) or vehicle alone (⊄), and this procedure was repeated 24 hrs later. Five days after the first sensitization, the mice were challenged with 0.2% DNFB on earlobe. Then the mice were exposed to a second DNFB challenge 3 weeks after the first challenge, and earlobe thickness was measured thereafter. (A) Net increase of thickness is shown as the mean +/− SEM (n=5). **P< 0.01 between the mice treated with placental extract implantation and those with sham operation. (B) Two days after the second DNFB challenge, earlobe tissues were harvested, processed, and stained with H&E. Representative fields of the tissues are shown. (C) Two days after the second DNFB challenge, spleen and PBMC were harvested from mice, stained with anti-CD4 and anti-CD62L mAbs, and analyzed by flow cytometry. The numbers indicate percentages of cells in the corresponding quadrants. Data are representative from 3 independently repeated experiments.