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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Biomaterials. 2010 Aug 16;31(33):8642–8650. doi: 10.1016/j.biomaterials.2010.07.085

Figure 8. Two-tier gradient with covalently coupled GDNF.

Figure 8

Microspheres were formed above the cloud point at 70°C. Dylight-488 (green) labeled GDNF was covalently coupled to PEG precursors during microsphere formation. The PEG/protein microspheres were incubated above the cloud point for 45 minutes (dye is covalently coupled to protein). Dylight-633 (blue) labeled microspheres were incubated above the cloud point for 11 minutes (no protein is coupled; dye is covalently coupled to PEG precursor). (a) Combined channels, (b) blue only, (c) green only.