Skip to main content
. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Biomaterials. 2010 Aug 16;31(33):8642–8650. doi: 10.1016/j.biomaterials.2010.07.085

Figure 9. Two-tier gradient with covalently coupled BSA/GDNF formed at 37°C.

Figure 9

Microspheres were formed above the cloud point at 37°C. Dylight-488 (green) labeled BSA/GDNF was covalently coupled to PEG precursors during microsphere formation. The PEG/protein microspheres were incubated above the cloud point for 65 minutes (dye is covalently coupled to protein). Dylight-633 (blue) labeled microspheres were incubated above the cloud point for 20 minutes (no protein coupled, dye bound to PEG precursor). (a) Combined channels, (b) blue only, (c) green only.